How to Neutralize Pet Odor in Subflooring

Pet urine can leave a stubborn, unpleasant smell that’s tough to eliminate. While many pet owners know how to treat stained carpets using special cleaners, urine often seeps through the carpet and soaks into the subflooring beneath. This hidden layer can trap odors and stains that regular carpet cleaning won’t remove. If your efforts haven’t eliminated the smell, try these simple steps to seal and neutralize pet odors in your subflooring.


What You’ll Need:

  • Oil-based stain-blocking primer (like Kilz Original Oil-Based Stain Blocking Primer)

  • Paintbrush

  • Face mask

  • Gloves

  • Shop vacuum


Steps to Neutralize Pet Urine in Subflooring:

  1. Clean the carpet around the stained area as best as possible.

  2. Safety first: Before starting, put on gloves and a face mask to protect yourself.

  3. Remove the carpet over the affected area and inspect the subfloor. Ensure it’s dry and free from mold. If you find mold or damp, rotting wood, contact a professional for help.

  4. Use a shop vacuum to remove any loose dirt or debris from the subfloor.

  5. Shake and stir your oil-based stain-blocking primer thoroughly.

  6. Using the paintbrush, apply the primer generously over the stained subfloor area.

  7. Allow the primer to dry completely.

  8. Reinstall your carpet.


Once sealed, your subfloor will no longer trap pet odors — giving you a fresher, cleaner home environment.

For the best results, combine this with a professional cleaning service like Molly Maid to keep your entire home smelling fresh and clean. Request a free estimate from your local Molly Maid today!